TAGMe DNA Methylation Detection Kits(qPCR) for Cervical Cancer

Short Description:

This product is used for in vitro qualitative detection of hypermethylation of the gene PCDHGB7 in cervical specimens.

Test method: Fluorescence quantitative PCR technology

Sample type: Female cervical specimens

Packing specification: 48 tests/kit

Product Detail

Product Tags




Validated over 36000 clinical samples in double-blind multi-center studies, the product has a specificity of 94.3% and a sensitivity of 96.0%.



The original Me-qPCR methylation detection technology can be completed in one step within 3 hours without bisulfite transformation.



Cervical cancer screening can be advanced to the stage of high-level lesions (precancerous lesions) stage.



Accompanied by customized result analysis software, the interpretation of the results is automated and directly readable.


This kit is used for in vitro qualitative detection of hypermethylation of the gene PCDHGB7 in cervical specimens. A positive result indicates an increased risk of grade 2 or higher-grade/more advanced cervical intraepithelial neoplasia (CIN2+, including CIN2, CIN3, adenocarcinoma in situ, and cervical cancer), which requires further colposcopy and/or histopathological examination. On the contrary, negative test results indicate that the risk of CIN2+ is low, but the risk cannot be completely excluded. Final diagnosis should be based on colposcopy and/or histopathological results. PCDHGB7 is a member of protocadherin family γ gene cluster. Protocadherin has been found to regulate biological processes such as cell proliferation, cell cycle, apoptosis, invasion, migration and autophagy of tumor cells through various signaling pathways, and its gene silencing caused by hypermethylation of the promoter region is closely related to the occurrence and development of many cancers. It has been reported that hypermethylation of PCDHGB7 is associated with a variety of tumors, such as non-Hodgkin lymphoma, breast cancer, cervical cancer, endometrial cancer and bladder cancer.


This kit contains nucleic acid extraction reagent and PCR detection reagent. Nucleic acid is extracted by magnetic-bead-based method. This kit is based on the principle of fluorescence quantitative PCR method, using methylation-specific real-time PCR reaction to analyze template DNA, and simultaneously detect the CpG sites of PCDHGB7 gene and the quality control marker internal reference gene fragments G1 and G2. The methylation level of PCDHGB7 in the sample, or the Me value, is calculated according to the PCDHGB7 gene methylated DNA amplification Ct value and the Ct value of the reference. The PCDHGB7 gene hypermethylation positive or negative status is determined according to the Me value.


Application scenarios

Early screening

Healthy people

Cancer Risk Assessment

High-risk population (positive for high-risk human papillomavirus (hrHPV) or positive for cervical exfoliation cytology)

Recurrence Monitoring

Postoperative population (with a history of high-grade cervical lesions or cervical cancer)

Clinical significance

Early screening for healthy population: Cervical cancer and precancerous lesions can be accurately screened out

Risk assessment in high-risk population: Risk classification can be performed in HPV-positive populations to guide subsequent triage detection

Recurrence monitoring for postoperative population: Postoperative population recurrence monitoring can be performed to prevent delays in treatment caused by recurrence

Sample collection

Sampling method: Place the disposable cervical sampler at the cervical os, gently rub the cervical brush and rotate 4-5times clockwise, slowly remove the cervical brush, put it into cell preservation solution, and label it for the following examination.

Preservation of samples: Samples can be stored at room temperature for up to 14 days, at 2-8 ℃ for up to 2 months, and at -20±5℃ for up to 24 months.

Detection process:3 Hours (Without manual process)

S9 Flyer small file

DNA Methylation Detection Kits (qPCR) for Urothelial Cancer


Clinical application

Clinical auxiliary diagnosis of cervical cancer

Detection gene


Sample type

Female cervical specimens

Test method

Fluorescence quantitative PCR technology

Applicable model


Packing specification

48 tests/kit

Storage Conditions

Kit A should be stored at 2-30℃

Kit B should be stored at -20±5℃

Valid for up to 12 months

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