Nucleic Acid Extraction Kit (A01)
Detection principle
After releasing the genomic DNA by splitting cells with the lysis buffer, the magnetic bead could selectively bind to the genomic DNA in the sample. A small number of impurities that are absorbed by the magnetic bead could be removed by the wash buffer. In TE, the magnetic bead could release the boundgenome DNA, obtaining the high-quality genome DNA. This method is simple and quick and the extracted DNA quality is high, which could meet the requirement for the detection of DNA methylation. Meanwhile, the extraction kit based on the magnetic bead could be compatible with automatic nucleic acid extraction, meeting the high-throughput nucleic acid extraction tasks.
Main components of the reagent
The components are shown in the table 1:
Table 1 Reagent Components and Loading
|
Component name |
Main components |
Size (48) |
Size (200) |
|
1. Lysis solution |
Guanidine hydrochloride, Tris |
11 mL/bottle |
44 mL/bottle |
|
2. Cleaning solutions A |
NaCl, Tris |
11 mL/bottle |
44 mL/bottle |
|
3. Cleaning solutions B |
NaCl, Tris |
13 mL/bottle |
26.5mL/bottle *2 |
|
4. Eluent |
Tris, EDTA |
12 mL/bottle |
44 mL/bottle |
|
5. Protease K solution |
Protease K |
1.1mL/piece |
4.4mL/piece |
|
6. Magnetic bead suspension 1 |
Magnetic beads |
1.1mL/piece |
4.4mL/piece |
|
7. Instructions to extracting nucleic acid reagents |
|
1 copy |
1 copy |
Components that are required in the nucleic acid extraction, but are not included in the kit:
1. Reagent: Anhydrous ethanol, isopropanol and PBS;
2. Consumables: 50ml centrifuge tube and1.5ml EP tube;
3. Equipment: Water bath kettle, pipettor, magnetic shelf, centrifuge, 96-hole deep plate (automatic), automatic nucleic acid extraction equipment (automatic).
Basic information
Sample requirements
1. The cross contamination between samples should be avoided in the sample collection and storage.
2.The detection shall be completed under the 7-day storage of ambient temperature after the collection of the cervical exfoliated cell sample (non-fixed). The detection shall be completed under the 30-day storage of ambient temperature after the collection of the urine specimen; The detection shall be completed in time after the collection of the cultured cell samples.
Parking specification: 200 pcs/box, 48 pcs/box.
Storage conditions: 2-30℃
Period of validity: 12 months
Applicable device: Tianlong NP968-C nucleic acid extraction instrument, Tiangen TGuide S96 nucleic acid extraction instrument, GENE DIAN EB-1000 nucleic acid extraction instrument.
Medical device record certificate No./product technical requirement No.: HJXB No. 20210099.
Date of approval and modification of the instructions:
Approval date: Nov. 18, 2021
